Cell Imaging Facility 

Department of Oncology    
Cross Cancer Institute      
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The Facility focuses on fluorescence microscopy and transmission microscopy techniques to local researchers. Here is a brief list of technologies researchers use in the Facility:

  1. General light microscopy services: phase, DIC, mulit-color fluorescence microscopy.

  2. Advanced fluorescence microscopy technology:
    1. Multi-dimensional (fluorescence) time-lapse microscopy.
    2. Deconvolution microscopy.
    3. Molecular interaction techniques (FRET (Fluorescence Resonance Energy Transfer); FCS (Fluorescence Correlative Spectroscopy);
    4. Photomanipulations:  Photoactivation/switching; FRAP (Fluorescence Recovery After Photobleaching), FLIP, laser microdissection or trapping, etc.
    5. Fluorescence lifetime Imaging (FLIM).
    6. Spectral imaging.

  3. Super resolution microscopy - STED

  4. Transmission electron microscopy - bright field, dark field.

  5. Advanced TEM microscopy:
    1. Tomography,
    2. CryoTEM: high pressure sample freezing, freeze substitution, Cryothin sectioning, CryoTEM observation
    3. Immuno-gold labeling,
    4. Electron Energy Loss Spectroscopy

  6. Microinjection

 

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