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Research Goal:
To study the role that
the transcription factor AP-2 plays in the developing retina. We
have found that AP-2 is expressed in retinal cells that are
differentiating along the amacrine and horizontal cell lineages.
We propose that AP-2 plays a key role in the transcriptional
regulation of genes required for amacrine and horizontal cell
function. The chromatin immunoprecipitation (ChIP) method is
being used to identify AP-2 target genes |
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Techniques: chromatin
immunoprecipitation, gel shift assays, in situ hybridization, DNA
transfection of primary retinal cultures, RT-PCR
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Publications:
Li. X., Glubrecht, D.D. and Godbout, R. The AP-2 transcription factor induces
apoptosis in retinoblastoma cells. Genes Chromosomes Cancer 49: 819-830 (2010).
Li. X., Glubrecht, D.D., Mita, R. and Godbout, R. Expression of AP-2d in the developing chick retina. Dev. Dyn. 237: 3210-3221 (2008).
Liu, R.-Z., Li, X and Godbout, R. A novel fatty acid-binding protein (FABP) gene resulting from tandem gene duplication in mammals: transcription in rat retina and testis. Genomics 92: 436-445 (2008).
Moeller, M.J., Soofi, A., Braun, G.S., Li, X., Watzl, C., Kriz, W.
and Holzman, L.B. (2004). Protocadherin FAT1 binds Ena/VASP proteins
and is necessary for actin and cell polarization. EMBO J. 29:
3769-3779.
Li., X., Meriane, M., Triki, I., Shekarabi, M., Kennedy, T., Larose,
L. and Lamarche-Vane, N. (2002). The adaptor protein Nck-1 couples
the netrin-1 receptor DCC (deleted in colon cancer) to the activation
of the small GTPase Rac1 through an atypical mechanism. J. Biol.
Chem. 277: 37788-37797.
Li., X., Saint-Cyr-Proulx, E. Artories, K., and Lamarche-Vane, N.
(2002). Rac1 and Cdc42 but not Rho or Rho-kinase activities are
required for neurite outgrowth induced by the Netrin-1 receptor DCC
(deleted in colon cancer) in N1E-115 neuroblastoma cells. J. Biol.
Chem. 277: 15207-15214.
Brage, V. M.M., Bestson, M., Li, X. and Lamarche-Vane, N. (2000).
Activation of the small GTPase Rac1 is sufficient to disrupt cadherin-dependent
cell-cell adhesion in normal human keratinocyte. Mol. Biol. Cell 11:
3703-3721.
Sadekova, S., Lamarche-Vane, N., Li, X. and Beauchemin, N. (2000).
The CEACAM1-L glycoprotein associates with the actin cytoskeleton and
localizes to cell-cell contact through activation of Rho-like GTPase.
Mol. Biol. Cell 11: 65-77.
Recent poster presentations:
Li, X., Glubrecht, D.D. and Godbout, R. The AP2 transcription factor induces apoptosis in retinoblastoma cells. 2010 Alberta Cancer Foundation Research Conference, Banff, Alberta (November 8-10, 2010).
Li, X., Monckton, E.A., Glubrecht, D.D. and Godbout, R. AP-2d transcription factor in intra-retinal axon projection. Ophthalmology Research Day 2010, University of Alberta (May 28, 2010).
Li, X., Glubrecht, D.D. and Godbout, R. Expression of AP-2 in the Developing Retina. 3rd Annual Canadian Neuroscience Meeting, Vancouver BC (May 24-27, 2009).
Li. X., Glubrecht, D. and Godbout, R. Expression of AP-2d in developing retina and retinoblastoma. Alberta Cancer Research Institute Research Meeting, Banff, Alberta (November 25-27, 2008).
Li, X., Glubrecht, D and Godbout, R. Expression of AP-2g in the Developing
Chick Retina. The American Society for Cell Biology 48th Annual Meeting, San Francisco, CA (December 13-17, 2008). Published in Mol. Biol. Cell Suppl. 19 (531/B482).
Li, X.,
Kim, J-H., Sereda, C., Monckton, E.A. and Godbout, R. The role of
AP-2 in retinal development. 4th Canadian Developmental Biology
Conference, Banff, Alberta 2008
Li, X., Kim, J-H., Sereda, C., Monckton, E.A. and Godbout, R. The
role of AP-2 in retinal development. 37th Annual meeting of the
Society for Neuroscience, San Diego, CA 2007
Li, X., Kim,
J-H., Sereda, C., Monckton, E.A. and Godbout, R. The role of AP-2 in
retinal development. Alberta Cancer Board Annual Research Meeting,
Banff, Alberta 2006
Li, X., Kim, J.-H., Sereda, C., Monckton, E.A. and Godbout, R. The
role of AP-2 in retinal development. 3rd Canadian Developmental
Biology Conference, Mont-Tremblant, Quebec (April 6-9, 2006).
Li, X., Kim, J.-H., Sereda, C. Monckton, E. and Godbout, R. The role
of AP-2 in retinal development and in retinoblastoma. ACB Annual
Research Meeting, Banff, Alberta (2005).
Funding source:
Alberta Heritage Foundation for Medical Research
Alberta Cancer Foundation
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