Cell Imaging Facility 

Department of Oncology    
Cross Cancer Institute      
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Equipment | LSM710 | Multiphoton STED | Spinning Disc Confocal | DIMs | PALM | FCS | FLIM | TEM | Micro-Injection | HCS | Workstataions and Tools

PALM laser Catapulting system
This is a PALM CombiSystem mounted on a Zeiss Axiovert 200M microscope. The setup is used primarily for manipulating cells with laser or selectively picking up cells or sub-cellular structures for biochemical analysis (e.g. single cell PCR). The workstation consists of a pulsed nitrogen laser, which allows precise cutting or micro-dissection. It is also equipped with an infrared laser for trapping and positioning cells.

PALM example

Example applications for this system are:

  1. Select morphologically identified cells/fragment of cells in pathological sample to study the sample biochemically.
  2. Using the laser tweezers to physically position 2 cells together to study cell-cell interactions.
  3. Selective ablation of cells in living tissue or cell culture.

More information about the system can be found on the following web site:  http://www.zeiss.de/microdissection

PALM

 

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