Cell Imaging Facility 

Department of Oncology    
Cross Cancer Institute      
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Fluorescence Correlative Spectroscopy Module (Zeiss Confocor 2/3)
FCS is a spectroscopic technique for the study of molecular interactions in solution. FCS monitors the random motion of fluorescently labelled molecules inside a defined volume element irradiated by a focused laser beam. These fluctuations provide information on the rate of diffusion (diffusion time) of a particle and this, in turn, is directly dependent on the particle's mass. As a consequence, any increase in the mass of biomolecules, e.g. as a result of an interaction with a second molecule, is readily detected as an increase in the particle's diffusion time.

FCS example

Because FCS measurement is made through diffraction limited volume, FCS can be used to study molecule-molecule interactions in living cell. The system can be used to study:

  1. Receptor-ligand interactions
  2. Transcription factor-DNA interactions
  3. Lipid-protein interactions, etc.

The FCS system at the Facility is integrated part of the Multi-photon and the LSM710/Zeiss LSM NLO systems with all the laser lines for common fluorophores and 2 detectors which enable cross correlation analysis.

More information about the system can be found on the following web site: http://www.zeiss.com/de/micro/home_e.nsf/Contents-FrameDHTML/4ACDCCD6CFEBFAEFC1256CB7004C8822



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